Evaluation of superovulatory treatment with extract of equine hypophysis in creole mares

Abstract

It was evaluated the super-ovulatory answer in creole mares which were administered with equine hypophysis extract (EHE) in two different doses, compared to FSH of porcine origin (Folltropin V). Twenty cyclical Colombian creole mares were taken whose ages oscillated between 2 and 8 years (5,3 years on average) which were randomly selected and assigned to the following four groups: Group 1 also called control group (T1): This group was administered with 5cc of saline solution IM (a.m. , p.m.); Group 2 (T2): 6,25 mg of Foltropin- v (FSH-P) IM (a.m., p.m.); Group 3 (T3): 8,3mg EHE IM (a.m., p.m.); Group 4 (T4): 12,5 mg EHE IM (a.m., p.m.). The treatments started seven days after the detection of an ovulation by ultrasound, the 8th day 12,5mg PgF2alfa were administered intramuscular (IM) via. The treatment with EHE was canceled when most of the follicles reached a size ≥ 35 mm, at that point 2500 UI of hCG were administered IV (intravenous via), followed by artificial insemination. Between the 7th or 8th day the embryos were collected by means of intrauterine pumping. A one way variance analysis (ANOVA) was done specifying the follicular growing between the day of the beginning of treatment and the ovulation, the number of pre-ovulatory follicles sized ≥ 35mm, the number of treatment days and the quantity of embryos collected on each group. A rank test or Duncan multiple comparison was carried out to see differences between the groups, and the condition and the quality of the embryos on each kind of treatment to determine the viability was determined through a relation analysis. The results showed that T4 group developed a higher number of follicles ≥ 35 mm in comparison to the rest of the groups (p<0.005) in 7,4 days of treatment, higher rate of follicle growing (3,01mm/day), higher number of ovulations per treatment (2,8) and a higher number of collected embryos per mare (1,6). So, T4 showed the best results in comparison to the results of the other groups in this work.