Establishment of a Breeding Protocol for Obtaining Embryonic/Fetal Murine Specimens

Abstract

Scientific research uses animal models in experimental situations where it is controversial to research with human specimens; such is the case of research with embryonic/fetal samples, where strict reproductive protocols are required that enable the accurate calculation of gestational age and thus obtaining appropriate sampling. Mice are the most widely used experimental models, as they are easy to handle and are of reproductive efficiency. The purpose of this paper is to present a reproduction protocol that makes it possible to determine the day of pregnancy in <em>Mus musculus </em>biomodel with certainty, in order to obtain embryonic/fetal specimens.