Disposition of danofloxacin in plasma and edible tissues of broiler chickens

Abstract

This paper aimed to study the disposition of danofloxacin in broiler chickens in order to establish pharmacokinetic parameters in plasma and tissues and to estimate a withdrawal period. 42 adult chickens were divided into 14 groups of 3 individuals each, who received a single oral dose of 5 mg/kg of danofloxacin after a fasting period of 12 h before and 3 h post-administration. Each batch was sacrificed at pre-established times, and blood, muscle, and liver samples were obtained over a period of up to 120 h post-application. The assay consisted of the liquid-liquid extraction of analyte and its separation and quantification by high-performance liquid chromatography (HPLC). Time averages for plasma and tissue concentration were analyzed using the PK Solution software. Withdrawal period was calculated using the WT 1.4 program, based on maximum residue limits (MRL) of 200 and 400 μg/kg, established for chicken muscle and liver, respectively. Danofloxacin exhibits a rapid absorption, resulting in a Cmax of 1.1 μg/ml with a Tmax of 1 h t½β (h) was 7.64 6.16 and 12.77 in plasma, liver, and muscle, respectively a Vd of 5.51 L/kg tissue/plasma ratios of 0.48 and 6.61 in muscle and liver, respectively, and levels up to 72 h. Based on residual concentration analysis, a withdrawal period of 1.4 and 3.34 d was estimated for muscle and liver, respectively.