Abstract
Seroprevalence of the antibodies of Brucella canis and Brucella abortus in dogs was assessed using a cross-sectional survey in Anambra and Enugu States, Nigeria. A total of 123 Companion dogs made up of 65 clinic dogs, 34 slaughter dogs and 24 household dogs were screened. For B. abortus antibody assay, the collected serum was used for Rose Bengal plate test (RBPT), Serum agglutination test (SAT) and Solid Phase Immunoassay technique with Immunocomb® Canine Brucellosis Antibody Test Kit was used. Out of the 123 dogs screened, none was positive for Brucella abortus antibodies while 34 (27.7%) of the dogs screened were positive for B. canis antibodies. There was a significant association (P<0.05) between infection and sex, the infection was significantly higher (P<0.05) in female than male dogs. Prevalence was significantly higher (P<0.05) in Exotic breeds than in mixed and local dog breeds. There was no association (P>0.05) between infection and antibody titre levels in the different categories of dogs. However, there was significant association (P<0.05) between the presence of Brucella canis antibodies and free roaming of dogs. This study provides the first serological evidence of B. canis infection in dogs in Enugu and Anambra States. This shows that B. canis is endemic in both states, underscoring the need for further studies. Female dogs, exotic breeds and freely roaming dogs are at a higher risk of Brucella infection in the study area; therefore, preventive and control measures are strongly recommendedReferences
Ducrotoy, MJ, Bertu WJ, Ocholi RA, Gusi AM, Bryssinckx W, Welburn S, et al. Brucellosis as an emerging threat in developing economies: Lessons from Nigeria. PloS Negl Trop Dis 2014; 24, 8(7):e3008. https://doi.org/10.1371/journal.pntd.0003008
Díaz Aparicio E. Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus. Rev Sci Tech 2013; 32(1): 43-51.
Njeru J, Wareth G, Melzer F, Henning K, Pletz MW, Heller R, et al. Systematic review of brucellosis in Kenya: disease frequency in humans and animals and risk factors for human infection. BMC Pub Hlth 2016; 16: 853. https://doi.org/10.1186/s12889-016-3532-9
Hamidy MER, Amin AS. Detection of Brucella spp. in the milk of infected cattle, sheep, goats and camels by PCR. Vet J 2002; 163: 299-305
Cadmus SIB, Ijagbone IF, Oputa HE, Adesokan HK, Stack JA 2006. Serological survey of brucellosis in livestock animals and workers in Ibadan, Nigeria. Afr J Biomed Res 2006; 9:163-8
Zhang N, Zhou H, Huang DS, Guan P. Brucellosis awareness and knowledge in communities worldwide: A systematic review and meta-analysis of 79 observational studies. PLoS neglect trop dis 2019; 13(5): e0007366. https://doi.org/10.1371/journal.pntd.0007366
Scholz HC, Mühldorfer K, Shilton C, Benedict S, Whatmore AM, Blom J, et al. The Change of a Medically Important Genus: Worldwide Occurrence of Genetically Diverse Novel Brucella Species in Exotic Frogs. PLoS ONE 2016; 11(12): e0168872. https://doi.org/10.1371/journal.pone.0168872
Scholz HC, Hubalek Z, Sedlacek Vergnaud G, Tomaso H, Aldahouk S, Melzer F, et al. Brucella macroti sp. Isolated from the common vole Microtus arvalis. Int J Syst Evol Microb 2008; 58: 375-82
Olsen SC, Palmer MV. Advancement of knowledge of Brucella over the past 50 years. Vet Pathol. 2014;51(6):107689
Franc, K.A., Krecek, R.C., Häsler, B.N. et al. Brucellosis remains a neglected disease in the developing world: A call for interdisciplinary action. BMC Public Health 18, 125 (2018). https://doi.org/10.1186/s12889-017-5016-y
Tekle M, Legesse M, Edao BM, Ameni G, Mamo G. Isolation and identification of Brucella melitensis using bacteriological and molecular tools from aborted goats in the Afar region of north-eastern Ethiopia. BMC Microbiol 2019; 19: 108.Available from:https://doi.org/10.1186/s12866-019-1474-y
De Massis F, Zilli K, Di Donato G, Nuvoloni R, Pelini S, Sacchini L, D'Alterio N, Di Giannatale E. Distribution of Brucella field strains isolated from livestock, wildlife populations, and humans in Italy from 2007 to 2015. PloS one 2019; 14(3): e0213689. https://doi.org/10.1371/journal.pone.0213689
Gul ST, Khan A. Epidemiology and epizootology of brucellosis: A review. Pak Vet J 2007; 27: 145-51
Banerjee K, Bhalty MA. A survey of bovine brucellosis in Northern Nigeria (A preliminary communication). Bull Epiz Dis Afr 1970; 18: 333-335
Ocholi RA, Kwaga JP, Ajogi I, Bale J, Bertu WJ. Epidemiology, Problems and prospect for control of brucellosis in Nigeria. Vom J Vet Sci 2004; 1: 78-86
Agada CA, Mohammed J, Okoh AEJ, Ogugua JA (2018) Prevalence and risk factors associated with brucellosis among high-risk individuals in Lafia, Nasarawa state, Nigeria. Int J One Hlth 2018;4: 45-51
Esuruoso GO 1974. Bovine brucellosis in Nigeria. Vet. Rec 1974; 95(3): 54-58
Chukwu CC. 1987. Studies on seroprevalence of bovine brucellosis in Enugu and Nsukka, Nigeria. Zariya Vet 1987; 2: 25-30
Ocholi RA, Kalejaiye JO, Okewole PA. Brucellosis in Nigeria. A review. Trop Vet 1993; 11: 15-26
Junaidu AU, Oboegbulem SI, Sharubutu GH, Daneji AI. Brucellosis in camels (Camelus dromedaries) slaughtered in Sokoto, Northwestern Nigeria. Anim Prod Res Adv 2006; 2: 15860
Junaidu AU, Oboegbulem SI, Salihu MD. Seroprevalence of brucellosis in prison farm in Sokoto State, Nigeria. Asian J Epidemiol 2008; 1(1): 24-8
Onunkwo JI, Njoga EO, Nwanta JA, Shoyinka SVO, Onyenwe IW, Eze JI. Serological survey of Porcine Brucella infection in South East, Nigeria. Nig Vet J 2011; 32: 60-62.
Kauffman LK, Bjork JK, Gallup JM, Boggiatto PM, Bellaire BH, Petersen CA 2014. Early detection of Brucella canis via quantitative polymerase chain reaction analysis. Zoon Pub Hlth 2014; 61(1): 48-54.
Lucero NE, Escobar GI, Ayala SM, Jacob N 2005. Diagnosis of human brucellosis caused by Brucella canis. J Med Microbiol 2005; 54: 457-61
Cadmus SIB, Adesokan HK, Ajala OO, Odetokun WO, Perrett LL, Stack JA. Seroprevalence of Brucella abortus and B. canis in household dogs in southwestern Nigeria: A preliminary report. J South Afr Vet Assoc 2011; 82: 5657
Behzadi MA, Mogheiseh A. Outbreak investigation of brucellosis at a Kennel in Iran. Pak Vet J 2011; 31: 379-380
Awah-Ndukum J, Mouiche MMM, Kouonmo-Ngnoyum L, Bayang HN, Manchang TK, Poueme RSN, Kouamo J, Ngu-Ngwa V, Assana E, Feussom KJM, Zoli AP. Seroprevalence and risk factors of brucellosis among slaughtered indigenous cattle, abattoir personnel and pregnant women in Ngaoundéré, Cameroon. BMC Infect Dis 2018; 18(1):611. https://doi.org/10.1186/s12879-018-3522-x
National Population Commission. Federal Republic of Nigeria Official Gazette, 2009, Abuja; 2006
Thursfield, M. Veterinary epidemiology. 3rd ed. Oxford: Blackwell; 2007
Adedoyin FJ, Adesokan HK, Otuh PI, Omobowale TO, Abiola JO, Stack JA et al. Sero-Prevalence of Brucellosis in Household and Stray Dogs in Ibadan, South Western Nigeria. Document taken from Proceedings of the 49th annual congress of the Nigerian Veterinary Medical Association; 2012 Nov 5-9; Ado- Ekiti, Ekiti State, Nigeria
Alton GG, Jones LM, Pietx DE 1975. Laboratory techniques in Brucellosis (Monograph Series No. 55). 2nd Ed. Geneva: FAO/WHO; 1975
Morgan WJB, Mackinon DJ, Gill KPW, Gover SGM, Norris PW. Standard laboratory techniques for the diagnosis of brucellosis. 2nd Ed. MAF. Weybridge, England: Central Veterinary Laboratory; 1978
Sati SN 2002. Seroepidemiological survey of Brucella abortus infection in Fulani breeding herds and trade cattle in the middle belt and southeast. (Ph.D thesis). Nsukka: University of Nigeria, Nsukka; 2002
Muhairwa AP, Mwakijungu EO, Msoffe PLM, Mtambo MMA. Seroprevalence and factors affecting canine monocytic ehrlichiosis and canine brucellosis in Tanzania. Res Opin Anim Vet Sci 2012; 2: 181188
Chessbrough M. District Laboratory Practice in Tropical Countries, Part 2. Cambridge Low Price Edition, Cambridge: University Press; 2000
Adesokan HK, Alabi PI, Ogundipe MA 2016. Prevalence and predictors of risk factors for Brucellosis transmission by meat handlers and traditional healers' risk practices in Ibadan, Nigeria. J Prev Med Hyg 2016; 57(3): E164-71.
Palomarez Ressendiiz E, Arellano Reynoso B, Hernandez Castro R, Tenorio Gutierrez V, SalasTellez E, SuarezGuemes F, Diaz-Aparicio E. Immunogenic Response of Brucella canis virB10 and virB11 Mutant in Murine Model. Front Cell Infect Microbiol 2012; 2:35. https://doi.org/10.3389/fcimb.2012.00035
Momoh HA, Ijale GO, Ajogi I, Okolocha EC. Seroprevalence of Canine Brucellosis in Jos, Plateau State, Nigeria. Asian J Epidemiol 2014; 7(2): 36-42
Radostits OM, Gay CC, Hinchcliff KW, Constable PD. Veterinary Medicine. A textbook of the diseases of cattle, horses, sheep, pigs, and goats. Brucellosis. 10th ed., Edinburgh, London, New York, Oxford, Philadelphia, St. Louis, Sydney, Toronto: Saunders Elsevier; 2007
Adesiyun AA, Adbullahi SU, Adeyanju JB. Prevalence of Brucella abortus and brucella canis antibodies in dogs in Nigeria. J Small Anim Pract 1986; 27: 31-7
Abubakar M, Mansoor M, Arshed MJ. Bovine brucellosis: old and new concepts with Pakistan perspective. Pak Vet J 2012; 32: 147-55
Aulakh HK, Patil PK, Sharma S, Kumar H, Mahajan V, Sandhu KS. A Study on the Epidemiology of Bovine Brucellosis in Punjab (India) Using Milk-ELISA. Acta Vet Brno 2008; 77: 393-9
Abukakar M, Arshed MJ, Hussain M, Ehtisham-Ul-Haq, Ali Q. Serological evidence of Brucella abortus prevalence in Punjab province, Pakistan-a cross-sectional study. Transbound Emerg Dis 2010; 57: 443-7
Escobar GI, Boeri EJ, Ayala SM, Lucero NE. The feasibility of using antigens prepared with rough Brucella strains for diagnosis of canine brucellosis. Rev Argent Microbiol 2010; 42(1): 35-40.
Johnson CA, Carter TD, Dunn JR, Baer SR, Schalow MM, Bellay YM, Guerra MA, Frank NA. Investigation and characterization of Brucella canis infections in pet-quality dogs and associated human exposures during a 20072016 outbreak in Michigan. J Amer Vet Med Assoc 2018; 253(3): 322-36
Keid LB, Soares RM, Vasconcellos SA, Megid J, Salgado VR, Richtzenhain LJ. Comparison of agar gel immunodiffusion test, rapid slide agglutination test, microbiological culture and PCR for the diagnosis of canine brucellosis. Res Vet Sci 2009; 86(1):22-6.
Chinyoka S, Dhliwayo S, Marabini L, Dutlow K, Matope G, Pfukenyi DM. Serological survey of Brucella canis in dogs in urban Harare and selected rural communities in Zimbabwe. J South Afr Vet Assoc 2014; 85(1): https://doi.org/10.4102/jsava. v85i1.1087
Keywords
Brucella abortus
Brucella canis
Canine brucellosis
risk factors
seroprevalence
zoonosis