Histological and Histochemical Description of the Pancreas in the Adult Lesser Capybara (Hydrochoerus isthmius)

Abstract

The aim of this study was to describe histologically and histochemically the pancreas of three adult Hydrochoerus isthmius, attacked by feral dog in Buenavista, Córdoba, Colombia. A complete necropsy was performed and pancreatic fragments were collected and stored in 10% buffered formalin and Bouin liquid. Then, they were dehydrated, diaphanized and embedded in paraffin, stained with Hematoxylin & Eosin, P.A.S., Gomori trichrome, Grimelius and Masson Fontana modified. The H. isthmius pancreas presented a duodenal mesenteric pattern. The exocrine portion was described as a composite tubuloacinar gland. The endocrine portion of the pancreas was constituted by pancreatic islets of Langerhans and a diffuse neuroendocrine system. The histological and histochemical techniques used allowed us to identify the exocrine and endocrine portion of the organ. It is suggested to complement this study with some special techniques for the identification of specific endocrine cells, such as Beta, Alpha, Delta, Epsilon, PP or Gamma.
PDF (Spanish)

References

Delgado C, Emmons L. Hydrochoerus isthmius. IUCN Red List of Threatened Species. 2016. Available at: https://www.iucnredlist.org/en

Mones A. Monografía de la familia Hydrochoeridae (Mammalia: Rodentia). Schweizerbart’sche Verlagsbuchhandlung; 1991. Available at: https://www.schweizerbart.de/publications/detail/isbn/9783510611331/Monografa_de_la_familia_Hydrochoeridae_Mammalia_Rodentia

Chacón J, Linares AJ, Ballesteros J, Carrascal J. Área de acción del chigüiro (Hydrochoerus Isthmius) en un sistema agropecuario en Córdoba, Colombia. Rev Colomb Cienc Anim. 2013;5(2): 270–281. Available at: https://doi.org/10.24188/recia.v5.n2.2013.291

Carrascal JC, Linares J, Chacón J. Comportamiento del Hydrochoerus hydrochaeris isthmius en un sistema productivo del departamento de Córdoba, Colombia. Rev MVZ Córdoba. 2011;6: 2754-2764. Available at: https://doi.org/10.21897/rmvz.276

Ojasti J. Ecology of capybara raising on inundated Savannas of Venezuela. Trop Eco Develop. 1980; 287-193.

Ballesteros-Correa J, Jorgenson JP. Aspectos poblacionales del cacó (Hydrochoerus hydrochaeris isthmius) y amenazas para su conservación en el noroccidente de Colombia. Mastozool Neotropical. 2009;16(1): 27-38.

Carrascal JC, Ortiz SA, César Fonseca C. Descripción histológica del hígado de Hydrochoerus hydrochaeris, Linnaeus, 1966. Rev Fac Cienc Vet. 2015;56(2): 67-71.

Carrascal JC. Aspectos histomorfométricos, histoquímicos e ultraestruturais do intestino grosso de capivaras (Hydrochoerus sp.) [doctorate thesis]. Viçosa, Brazil: Universidade Federal de Viçosa; 2017.

Takaki R, Ono J. Preparation and culture of isolated islets and dissociated islet cells from rodent pancreas. J Tissue Cult Methods. 1985;9(2): 61-66. Available at: https://doi.org/10.1007/BF01797776

Kelly CB, Blair LA, Corbett JA, Scarim AL. Isolation of islets of Langerhans from rodent pancreas. In: Diabetes mellitus: methods and protocols. Totowa, NJ: Humana Press; 2003. pp. 3-14.

Murakami T, Hitomi S, Ohtsuka A, Taguchi T, Fujita T. Pancreatic insulo-acinar portal systems in humans, rats, and some other mammals: Scanning electron microscopy of vascular casts. Microsc Res Tech. 1997;37(5-6): 478-88. Available at: https://doi.org/10.1002/(SICI)1097-0029(19970601)37:5/6<478::AID-JEMT10>3.0.CO;2-N

Carrascal JC. Histological and histochemical studies of the organs of the digestive tract, liver and pancreas of adult capybaras Hydrochoerus hydrochaeris Linnaeus, 1766 (Mammalia, Rodentia, Hydrochaeridae) [master thesis]. Viçosa, Brazil: Universidade Federal de Viçosa; 2001.

Panesi AG, Chávez RA, Navarrete ZM, Sandoval CN, Sato SA, Santos RF. Estudio morfológico del páncreas de alpacas (Vicugna pacos) jóvenes y adultas. Rev Investig Vet Perú. 2018;29(1): 75-83. Available at: https://doi.org/10.15381/rivep.v29i1.14082

Cebra CK, Bildfell RJ, Fischer KA. Microanatomic features of pancreatic islets and immunolocalization of glucose transporters in tissues of llamas and alpacas. Am J Vet Res. 2006;67(3): 524-528. Available at: https://doi.org/10.2460/ajvr.67.3.524

Roberts PF, Burns J. A histochemical study of mucins in normal and neoplastic human pancreatic tissue. J Pathol. 1972;107(2): 87-94. Available at: https://doi.org/10.1002/path.1711070203

Lev R, Spicer SS. A histochemical comparison of human epithelial mucins in normal and in hypersecretory states including pancreatic cystic fibrosis. Am J Pathol. 1965;46: 23-47.

Barnd DL, Lan MS, Metzgar RS, Finn OJ. Specific, major histocompatibility complex-unrestricted recognition of tumor-associated mucins by human cytotoxic T cells. Proc Natl Acad Sci USA. 1989;86(18): 7159-7163. Available at: https://doi.org/10.1073/pnas.86.18.7159

Treuting PM, Dintzis Z, Montine K. Comparative anatomy and histology [Internet]. 2nd ed. Seattle: Academic Press; 2017.

Motta PM, Macchiarelli G, Nottola SA, Correr S. Histology of the exocrine pancreas. Microsc Res Tech. 1997;37(5-6): 384-98. Available at: https://doi.org/10.1002/(SICI)1097-0029(19970601)37:5/6<384::AID-JEMT3>3.0.CO;2-E

Dolenšek J, Rupnik MS, Stožer A. Structural similarities and differences between the human and the mouse pancreas. Islets 2015;7(1): e1024405. Available at: https://doi.org/10.1080/19382014.2015.1024405

Baron M, Veres A, Wolock SL, Faust AL, Gaujoux R, Vetere A et al. A single-cell transcriptomic map of the human and mouse pancreas reveals inter- and intra-cell population structure. Cell Syst. 2016;3(4): 346-360. Available at: https://doi.org/10.1016/j.cels.2016.08.011

Officer B. Silver Stains: Grocott’s methenamine silver, Jone’s methenamine silver, and Masson Fontana. In: HIML251 lecture notes; 2009

Bressan MS, Fonseca CC, Menin E, de Paula T. Identificação e quantificação de gânglios nervosos, células argentafins, argirófilas e imunorreativas à serotonina no ceco da capivara (Hydrochoerus hydrochaeris). Ceres. 2004;51(298): 729-749.

Keywords

histology
digestive tract
exocrine
endocrine
rodent