Molecular detection of BLV in bovines and association with risk/protection factors in herds from Risaralda (Colombia)

Abstract

<p>Bovine leukosis is a disease caused by an oncogenic retrovirus that belongs to the Retroviridae family and is known for its worldwide distribution, causing great economic losses, especially in dairy farming. Few studies on the disease have been reported in Risaralda (Colombia). The objective was to determine the prevalence of the bovine leukosis virus and its association with some risk/protection factors in cattle herds from Risaralda. Blood samples were taken from 145 individuals from 6 herds in the Risaralda region, during the year 2018. DNA extraction was carried out by salting out and PCR was used to detect the provirus DNA. For each animal, the racial composition, sex, age group, origin and herd were taken in order to evaluate possible factors associated with the presence of the virus. A descriptive analysis of the information was performed, the prevalence was estimated with the 95% confidence interval, and the effect of risk/protection factors on leukosis positivity was determined by logistic regression and the odds ratio was estimated. All analyzes were done using R software. A general prevalence of 6.90% was estimated. Statistically significant differences were found for age group one (Under 5 years) and two (5 to 10 years) with respect to age group three (Over 10 years) (OR = 0.19 in both cases), indicating a higher frequency with age. This work is one of the first carried out in the department of Risaralda and provides surveillance of the disease in Colombia.</p>
PDF (Spanish)

References

García. MLR. Manejo de Enfermedades del ganado de carne y leche. Curso del INCAPA. 2010. 22–39 p.

Marawan MA, Alouffi A, El Tokhy S, Badawy S, Shirani I, Dawood A, et al. Bovine leukaemia virus: Current epidemiological circumstance and future prospective. Vol. 13, Viruses. 2021;13(11):2167. https://doi.org/10.3390/v13112167

Gutiérrez SE, Lützelschwab CM, Barrios CN, Juliarena MA. Leucosis bovina: una visión actualizada. Rev Inv Vet Perú. 2020;31(3):1–28. https://doi.org/10.15381/rivep.v31i3.16913

Gillet N, Florins A, Boxus M, Burteau C, Nigro A, Vandermeers F, et al. Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human. Retrovirology. 2007;4:18. https://doi.org/10.1186/1742-4690-4-18

Pozzatti PN, Valentim TP, Bissi B, Casagrande FP, Martins CB. Leucose enzoótica bovina: Revisão literária Pubvet. 2010;4(32).

Kohara J, Konnai S, Onuma M. Experimental transmission of Bovine leukemia virus in cattle via rectal palpation. Jpn J Vet Res. 2006;54(1):25–30.

OIE. Leucosis bovina enzoótica. En: Manual de las pruebas de diagnóstico y de las vacunas para los animales terrestres. Office International Des Epizooties; 2019. p. 12.

Santos MJD, Trono K, Lager I, Wigdorovitz A. Development of a PCR to diagnose BLV genome in frozen semen samples. Vet Microbiol. 2007;119(1):10–8. https://doi.org/10.1016/j.vetmic.2006.08.030

Wu D, Murakami K, Morooka A, Jin H, Inoshima Y, Sentsui H. In vivo transcription of bovine leukemia virus and bovine immunodeficiency-like virus. Virus Res. 2003;97(2):81–87. https://doi.org/10.1016/S0168-1702(03)00222-3

Motta-Delgado PA, Rivera-Calderón LG, Herrera-Valencia W, Martínez-Tovar RA, Londoño-Sánchez M, Rojas-Vargas EP, et al. Seroprevalencia del virus de la leucosis en bovinos del departamento del Caquetá, Colombia. Rev Colomb Cienc Anim - RECIA. 2019;11(2):722. https://doi.org/10.24188/recia.v11.n2.2019.722

Úsuga-Monroy C, Echeverri-Zuluaga JJ, López-Herrera A. Detección molecular y serológica del virus de la leucosis bovina en una población de vacas Holstein, de Colombia. Rev Mex Ciencias Pecu. 2018;9(2): 387–399. https://doi.org/10.22319/rmcp.v9i2.4232

Gao A, Kouznetsova VL, Tsigelny IF. Bovine leukemia virus relation to human breast cancer: Meta-analysis. Microb Pathog. 2020;149. https://doi.org/10.1016/j.micpath.2020.104417

Erskine RJ, Bartlett PC, Byrem TM, Render CL, Febvay C, Houseman JT. Association between bovine leukemia virus, production, and population age in Michigan dairy herds. J. Dairy Sci. 2012;95(2):727-734. https://doi.org/10.3168/jds.2011-4760

IDEAM. Atlas climatológico de Colombia [Internet]. 2023 [citado enero 16 de 2023]. http://atlas.ideam.gov.co/visorAtlasClimatologico.html

Miller SA, Dykes DD, Polesky HF. A simple salting–out procedure for extracting ADN from human nucleated cells. Nucleic Acids Res. 1988;16(3):1215. https://doi.org/10.1093/nar/16.3.1215

Polat M, Takeshima S nosuke, Hosomichi K, Kim J, Miyasaka T, Yamada K, et al. A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis. Retrovirology. 2016;13. https://doi.org/10.1186/s12977-016-0239-z

Hernández-Herrera DY, Posso-Terranova AM, Benavides JA, Muñoz-Flórez, Eduardo J, Giovambattista G, Álvarez-Franco LÁ. Detección del virus de la leucosis bovina en ganado criollo colombiano mediante PCR-anidado. Acta Agron. 2011;60(4):312–318.

Úsuga-Monroy C, Echeverri JJ, López-Herrera A. El componente racial influencia la resistencia a la infección con el virus de la leucosis bovina. Rev Fac Med Vet Zootec. 2018;65(2):130-139. https://doi.org/10.15446/rfmvz.v65n2.75632

Meza-Barreto G, Sanjuanelo-Corredor DW, Gallego-Marín MI. Detección molecular del virus de la leucosis bovina: un estudio por conglomerados en Colombia. Cienc y Agric. 2016;13(2):47–55. https://doi.org/10.19053/01228420.v13.n2.2016.5552

Ramírez Vásquez NF, Fernández Silva JA, Argaiz DV, Londoño Pino J, Chaparro Gutiérrez JJ, Olivera Ángel ME. Seroprevalence and risk factors of several bovine viral diseases in dairy farms of San Pedro de los Milagros, Antioquia, Colombia. Rev CES Med Vet y Zootec. 2016;11(1):15–25. https://doi.org/10.21615/cesmvz.11.1.2

Mariño O. Situación de la investigación en leucosis bovina en Colombia. Rev ACOVEZ. 1984;8(27):22–6.

Carrero Rojas JL, Arévalo Martínez F, Tarazona Suárez A, Cepeda BM. Prevalencia de la seropositividad a la leucosis bovina mediante la técnica diagnóstica de ELISA indirecta en hatos lecheros situados en Mesa de los Santos, Santander. Spei Domus. 2009;5(11):6-11.

Corredor-Figueroa AP, Salas S, Olaya-Galán NN, Quintero JS, Fajardo Á, Soñora M, et al. Prevalence and molecular epidemiology of bovine leukemia virus in Colombian cattle. Infect Genet Evol. 2020;80. https://doi.org/10.1016/j.meegid.2020.104171

Rincón Flórez JC, Peláez Peláez EA, Trejos Marín N, Echeverry López JC, González Corrales JC. Prevalencia del virus de la leucosis bovina en búfalos de agua en la región centro occidental de Colombia. Rev Mex Ciencias Pecu. 2021;12(2). https://doi.org/10.22319/rmcp.v12i2.5459

Lee EJ, Kim EJ, Ratthanophart J, Vitoonpong R, Kim BH, Cho IS, et al. Molecular epidemiological and serological studies of bovine leukemia virus (BLV) infection in Thailand cattle. Infect Genet Evol. 2016;41:245-254. https://doi.org/10.1016/j.meegid.2016.04.010

Hernández A. Evaluación de la resistencia genética del ganado criollo hartón del valle al virus de la leucosis bovina en infección natural. Aica [Internet]. 2014 [citado enero 16 de 2023]; 4: 3–5. https://shorturl.at/vIJK5

Betancur C, Rodas J. Seroprevalencia del virus de la Leucosis Viral Bovina en animales con trastornos reproductivos de Montería. Rev MVZ Cordoba. 2008;13(1). https://doi.org/10.21897/rmvz.411

Saldarriaga-Saldarriaga A, Londoño M, González-Herrera LG, Rincón JC, López-Herrera A. Seropositivity for bovine viral diarrhea and enzootic bovine leukemia viruses in Blanco Orejinegro cattle in Colombia and infection associated-factors. Rev Fac Med Vet y Zootec. 2021;68(2). https://doi.org/10.15446/rfmvz.v68n2.98031

Keywords

enzootic bovine leukosis
lymphosarcoma
PCR
bovine leukemia virus