Diversidade molecular de candidatos para vacina em Leptospira spp.

Abstract

The aim of this study was to determine the molecular diversity of OmpL1, LipL32, LipL41, LigA and LigB proteins and that of the genes that encode them using bioinformatic analysis in different pathogenic strains of <em>Leptospira</em> spp. based on the information available in databases. The amino acid sequences of OmpL1, LipL32, LipL41, LigA and LigB proteins were used, as well as the genes encoding them in strains of <em>Leptospira</em> spp. reported at The National Center for Biotechnology Information (NCBI). The analysis of proteins and genes were performed using the <em>Protein, Nucleotide</em> and <em>Gene</em> resources from the NCBI. The alignment of the consensus sequences was performed using the PSI-BLAST and BLASTn tools. The coverage percentage of the selected sequences of the <em>ompL1</em>, <em>lipL32</em>, <em>lipL41</em>, <em>ligA</em> and <em>ligB</em> genes in pathogenic strains of <em>Leptospira</em> spp. is 100% for <em>ompL1</em>, <em>lipL32</em> and <em>lipL41</em>, 75% for <em>ligA</em> and 99% for <em>ligB</em> with identity percentages of 85, 98, 88, 90 and 80% respectively the coverage percentage of the selected protein sequences is 10 77, 99, 100 and 100% with identity percentages of 9 99, 92, 63 and 60% respectively, indicating that genes and proteins, except LigA and LigB proteins, are highly conserved in various pathogenic serovars of <em>Leptospira</em> spp. According to these results, it is recommended that further analysis of these proteins be made in order to determine the feasibility of its use as vaccine candidates.