Larval Mortality in Culexquinquefasciatus and Anopheles albimanus (diptera: culicidae), Caused with a Bacillus sphaericus (bacteria: bacillaceae) Granulated Product in Experimental Conditions

Abstract

The purpose of this study was to assay a series of concentrations of a granulated product prepared with Bacillus sphaericus on Culexquinquefasciatus and Anopheles albimanus larvae in laboratory conditions. Eleven concentrations (2 4 6 8 10 12 14 16 18 20 500 ppm) were used on An. Albimanus larvae and eight concentrations ten times smaller (2, 4, 6, 8, 1 12, 14, 16 ppm) were used on Cx. quinquefasciatus larvae. Sixty (60) larvae and a control with 20 larvae were used per concentration. The time of exposure was of 48 hours at a laboratory temperature of 28 ± 2 ºC. LC50 and LC95 were determined through the Probit tests. A LC95 of B. sphaericus was found between 6,45 and 7,28 ppm for Cx. quinquefasciatus larvae; whereas LC95 was observed between 450.56 and 466.76 ppm in An. albimanus larvae.