Abstract
The Ewes Enzootic Abortion is a disease caused by Chlamydia abortus. It is deemed a zoonosis and one of the leading causes of financial losses in this type of business. This article focuses on using the culture of leukocytes from animals without any abortion symptoms and antibody detection to determine the potential presence of C. abortus in backyard exploitations. Forty-two samples of peripheral blood were obtained from ewes in different populations. The detection of Chlamydia abortus was carried out by using the Giemsa dye and PCR technique. Anti-C. Abortus antibody detection was performed through a commercial ELISA technique. Results showed 21 positive samples using the PCR, and only ten were positive according to the Giemsa dye, while 22 serum samples showed anti-C. abortus antibody. In this study, 38.1% of the samples were positive for the C. abortus infection, as verified with the PCR and serology. In conclusion, peripheral blood leukocytes can be helpful to detect an infection caused by Chlamydia spp. Animal exploitation without any previous abortion allows knowing the real prevalence of ewes’ enzootic abortion in Mexico.References
Cheong HC, Lee CYQ, Cheok YY, Tan GMY, Looi CY, Wong WF. Diseases in Primary Hosts and Zoonosis. Microorganisms. 2019;7(5): 146. Disponible en: https://doi.org/10.3390/microorganisms7050146
Palomares REG, Mejía SP, Aguilar RF, de la Cruz CL, Jiménez SH, Leyva CJC, et al. Frecuencia y factores de riesgo asociados a la presencia de Chlamydia abortus, en rebaños ovinos en México. Rev Mex Cienc Pecuarias. 2020;11(3): 783-794. Disponible en: https://doi.org/10.22319/rmcp.v11i3.5269
Jiménez-Estrada JM, Escobedo-Guerra MR, Arteaga-Troncoso G, López-Hurtado M, de Haro-Cruz MJ, Montes De Oca JR, et al. Detection of Chlamydophila abortus in sheep (Ovis aries) in Mexico. Am J Anim Vet Sci. 2008;3(4): 91-95. Disponible en: https://doi.org/10.3844/ajavsp.2008.91.95
Soriano-Vargas E, Jiménez-Estrada JM, Salgado-Miranda C, López-Hurtado M, Escobedo-Guerra MR, Guerra-Infante FM. Identificación de Chlamydophila abortus en un aborto ovino en Almoloya de Juárez, México. Redvet. 2011;12(11): 1-6. Disponible en: http://www.veterinaria.org/revistas/redvet/n111111/111104.pdf
Montes de Oca JR, Rodríguez DMC, Aldama FJ. Chlamydia abortus, Implicaciones en la veterinaria en el contexto mexicano. RMAE. 2021;8(1): 11-13.
Longbottom D, Livingstone M, Maley S, van der Zon A, Rocchi M, Wilson K, et al. Intranasal infection with Chlamydia abortus induces dose-dependent latency and abortion in sheep. PLoS One. 2013;8(2): e57950. Disponible en: https://doi.org/10.1371/journal.pone.0057950
Essig A, Longbottom D. Chlamydia abortus: new aspects of infectious abortion in sheep and potential risk for pregnant women. Curr Clin Micro R. 2015;2: 22–34. Disponible en: https://doi.org/10.1007/s40588-015-0014-2
Kuo CC. Cultures of Chlamydia trachomatis in mouse peritoneal macrophages: factors affecting organism growth. Infect Immun. 1978;20: 439–445. Disponible en: https://doi.org/10.1128/iai.20.2.439-445.1978
Guerra-Infante F, García-Zaragoza FJ, Arredondo-García JL. Sensibilidad y especificidad de los macrófagos peritoneales de ratón en el diagnóstico de infecciones por Chlamydia trachomatis. Rev Latin-Amer Microbiol. 1992;34(1): 7-10.
Rajaram K, Nelson DE. Chlamydia muridarum Infection of Macrophages Elicits Bactericidal Nitric Oxide Production via Reactive Oxygen Species and Cathepsin B. Infec Immun. 2015;83(8): 3164-3175. Disponible en: https://doi.org/10.1128/IAI.00382-15
Sachse K, Vretou E, Livingstone M, Borel N, Pospischil A, Longbottom D. Recent developments in the laboratory diagnosis of chlamydial infections. Vet Microbiol. 2009;135(1-2): 2-21. Disponible en: https://doi.org/10.1016/j.vetmic.2008.09.040
Everett KD, Andersen AA. Identification of nine species of the Chlamydiaceae using PCR-RFLP. Int J Syst Bacteriol. 1999;49: 803-813. Disponible en: https://doi.org/10.1099/00207713-49-2-803
Laroucau K, Trichereau A, Vorimore F, Mahe AM. A pmp genes-based PCR as a valuable tool for the diagnosis of avian chlamydiosis. Vet Microbiol. 2007;121: 150–157. Disponible en: https://doi.org/10.1016/j.vetmic.2006.11.013
Villagrana ZJR, López HM, Flores SVR, de Haro CMJ, Escobedo GMR, Guerra IFM. Persistence of Chlamydia trachomatis in endometrium and peritoneal fluid of infertile patients with negative cervical culture. Ginecol Obstet Mex. 2013;81(1): 23-28.
Menzies PI. Causes of infectious abortion in sheep and goats. Vet Clin North Am Food Anim Pract. 2011;27(1): 81-93. Disponible en: https://doi.org/10.1016/j.cvfa.2010.10.011
López-Hurtado M, Cuevas-Recillas KN, Flores-Salazar VR, Guerra-Infante FM. Chlamydia trachomatis DNA in leukocytes of peripheral blood from neonates. Enferm Infecc Microbiol Clin. 2015;33(7): 458–463. Disponible en: https://doi.org/10.1016/j.eimc.2014.09.019
Cirino F, Webley WC, West C, Croteau NL, Andrzejewski C, Stuart ES. Detection of Chlamydia in the peripheral blood cells of normal donors using in vitro culture, immunofluorescence microscopy and flow cytometry techniques. BMC Infect Dis. 2006;6: 23. Disponible en: https://doi.org/10.1186/1471-2334-6-23
Osman KM, Ali HA, ElJakee JA, Galal HM. Chlamydophila psittaci and Chlamydophila pecorum infections in goats and sheep in Egypt. Rev Sci Tech. 2011;30(3): 939-948. Disponible en: https://doi.org/10.20506/rst.30.3.2088
Buxton D, Barlow RM, Finlayson J, Anderson IE, Mackellar A. Observations on the pathogenesis of Chlamydia psittaci infection of pregnant sheep. J Comp Pathol. 1990;102: 221–237. Disponible en: https://doi.org/10.1016/S0021-9975(08)80127-2
Anderson IE, Baxter SI, Dunbar S, Rae AG, Philips HL, Clarkson MJ, et al. Analyses of the genomes of chlamydial isolates from ruminants and pigs support the adoption of the new species Chlamydia pecorum. Int J Syst Bacteriol. 1996;46(1): 245-251. Disponible en: https://doi.org/10.1099/00207713-46-1-245
Longbottom D, Coulter LJ. Animal chlamydioses and zoonotic implications. J Comp Pathol. 2003;128(4): 217-244. Disponible en: https://doi.org/10.1053/jcpa.2002.0629
Keywords
Chlamydia abortus
leukocytes
seroprevalence