Uso y diferencias del concentrado autólogo de plaquetas y fibrina rica en plaquetas en veterinaria
Los productos derivados de plaquetas (PDP), como el concentrado autólogo en plaquetas (CAP) y la fibrina rica en plaquetas (FRP), han despertado gran interés en la medicina por su capacidad de regenerar tejidos duros y blandos. Se obtienen a partir de la sangre del paciente y han sido utilizados en diversas especialidades quirúrgicas, incluidas la cirugía oral y maxilofacial. Estudios han evaluado su eficacia en enfermedades osteomusculares y heridas, con resultados prometedores en la regeneración y cicatrización. El CAP es una preparación de plasma autólogo con alta concentración de plaquetas y factores de crecimiento, obtenida mediante centrifugación para eliminar los glóbulos rojos. La FRP, por su parte, actúa como un andamio celular que favorece la migración celular y la curación de tejidos, sin necesidad de aditivos en su obtención. En medicina veterinaria, la aplicación del CAP y la FRP ha mostrado efectos positivos en la curación de defectos óseos y musculares en modelos animales. Sin embargo, a pesar de los resultados alentadores, su uso no está completamente aceptado, ya que la mayoría de los estudios han sido realizados en poblaciones pequeñas y su aplicabilidad aún no ha sido evaluada a gran escala. Se requiere más investigación para comprender mejor sus efectos y garantizar su seguridad y eficacia en diferentes especies y patologías. Este trabajo recopila estudios recientes en modelos animales sobre el uso del CAP y FRP en medicina veterinaria, en función de destacar sus efectos positivos en la curación de defectos óseos, musculares y otras lesiones.
Feigin K, Shope B. Use of platelet-rich plasma and platelet-rich fibrin in dentistry and oral surgery: Introduction and review of the literature. J Vet Dent. 2019 jun.;36(2):109-123. https://doi.org/10.1177/0898756419876057
Soares CS, Babo PS, Reis RL, Carvalho PP, Gomes ME. Platelet-derived products in veterinary medicine: A new trend or an effective therapy? Trends in Biotechnol. 2021 mzo.;39(3), 225-243. https://doi.org/10.1016/J.TIBTECH.2020.07.011
Perinelli DR, Bonacucina G, Pucciarelli S, Cespi M, Serri E, Polzonetti V, et al. Rheological properties and growth factors content of platelet-rich plasma: Relevance in veterinary biomedical treatments. Biomedicines. 2020 oct. 18;8(10): 429. https://doi.org/10.3390/biomedicines8100429
Gonçalves NJ, Frantz N, Oliveira, RM de. Platelet-rich plasma (PRP) therapy: An approach in reproductive medicine based on successful animal models. Anim Reprod. 2020 may. 22;16(1):93-98. https://doi.org/10.21451/1984-3143-ar2018-093
Dohan-Ehrenfest DM, Bielecki T, Mishra A, Borzini P, Inchingolo F, Sammartino G, et al. In search of a consensus terminology in the field of platelet concentrates for surgical use: platelet-rich plasma (PRP), platelet-rich fibrin (PRF), fibrin gel polymerization and leukocytes. Curr Pharm Biotechnol. 2012 jun.;13(7):1131-1137. https://doi.org/10.2174/138920112800624328
Song D, Huang Y, Van Dessel J, Shujaat S, Orhan K, Vangansewinkel T, Van den Eynde K, et al. Effect of platelet-rich and platelet-poor plasma on peri-implant innervation in dog mandibles. Int J Implant Dent. 2019 dic. 4;5(1):40. https://doi.org/10.1186/S40729-019-0193-3
Chun N, Canapp S, Carr BJ, Wong V, Curry J. Validation and characterization of platelet-rich plasma in the feline: A prospective analysis. Front Vet Sci. 2020 ag. 11;7:512. https://doi.org/10.3389/fvets.2020.00512
Ferrari JT, Schwartz P. Prospective evaluation of feline sourced platelet-rich plasma using centrifuge-based systems. Front Vet Sci. 2020 jun. 12;7:322. https://doi.org/10.3389/fvets.2020.00322
Cengiz IF, Oliveira JM, Reis RL. PRP Therapy. Adv Exp Med Biol. 2018;1059:241-253. https://doi.org/10.1007/978-3-319-76735-2_11
Camargo-Garbin L, Lopez C, Carmona JU. A critical overview of the use of platelet-rich plasma in equine medicine over the last decade. Front Vet Sci. 2021 mar. 31;(8):641818. https://doi.org/10.3389/fvets.2021.641818
Camargo-Garbin L, Olver CS. Platelet-rich pro¬ducts and their application to osteoarthritis. J Equine Vet Sci. 2020 mar.;86:102820. https://doi.org/10.1016/j.jevs.2019.102820
Wu PIK, Diaz R, Borg-Stein J. Platelet-rich plasma. Phys Med Rehabil Clin N Am. 2016 nov.;27(4):825-853. https://doi.org/10.1016/j.pmr.2016.06.002
Marx RE. Platelet-rich plasma (prp): What is PRP and what is not PRP? Implant Dent. 2001;10(4):225- 228. https://doi.org/10.1097/00008505-200110000-00002
Ortved KF. Regenerative medicine and rehabilitation for tendinous and ligamentous injuries in sport horses. Vet Clin North Am Equine Pract. 2018 ag.;34(2):359-373. https://doi.org/10.1016/j.cveq.2018.04.012
Kornsuthisopon C, Pirarat N, Osathanon T, Kalpravidh C. Autologous platelet-rich fibrin stimulates canine periodontal regeneration. Sci Rep. 2020 feb. 5;10(1):1850. https://doi.org/10.1038/S41598-020-58732-X
Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler, C, Dohan, SL, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part iv: Clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 mar.;101(3):e56-e60. https://doi.org/10.1016/j.tripleo.2005.07.011
Karimi K, Rockwell H. The benefits of platelet-rich fibrin. Facial Plast Surg Clin North Am. 2019 ag.;27(3):331-340. https://doi.org/10.1016/J.FSC.2019.03.005
Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJJ, Mouhyi J, et al. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part II: Platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006 mar.;101(3):e45-e50. https://doi.org/10.1016/j.tripleo.2005.07.009
Fan Y, Perez K, Dym H. Clinical uses of platelet-rich fibrin in oral and maxillofacial surgery. Dent Clin North Am. 2020 abr.;64(2):291-303. https://doi.org/10.1016/j.cden.2019.12.012
Oryan A, Alidadi S, Moshiri A. Platelet-rich plasma for bone healing and regeneration. Expert Opin Biol Ther. 2016;16(2):213-232. https://doi org/10.1517/14712598.2016.1118458
Marx RE. Platelet-rich plasma: Evidence to support its use. J Oral Maxillofac Surg. 2004 abr.;62(4):489-496. https://doi.org/10.1016/j.joms.2003.12.003
Rezaei M, Jamshidi S, Saffarpour A, Ashouri M, Rahbarghazi R, Reza-Rokn A, et al. Transplantation of bone marrow-derived mesenchymal stem cells, platelet-rich plasma, and fibrin glue for periodontal regeneration. Int J Periodontics Restorative Dent. 2019 ene.;39(1):e32-e45. https://doi.org/10.11607/prd.3691
Iacopetti I, Patruno M, Melotti L, Martinello T, Bedin S, Badon T, et al. Autologous platelet-rich plasma enhances the healing of large cutaneous wounds in dogs. Front Vet Sci. 2020 oct. 26;7:575449. https://doi.org/10.3389/fvets.2020.575449
Rashid A, ElSharaby FA, Nassef, EM, Mehanni S, Mostafa YA. Effect of platelet-rich plasma on orthodontic tooth movement in dogs. Orthod Craniofac Res. 2017 may.;20(2):102-110. https://doi.org/10.1111/OCR.12146
Gad SB, Hafez MH, El-Sayed YS. Platelet-rich plasma and/or sildenafil topical applications accelerate and better repair wound healing in rats through regulation of proinflammatory cytokines and co-llagen/tgf-β1 pathway. Environ Sci Pollut Res Int. 2020 nov.;27(32):40757-40768. https://doi.org/10.1007/S11356-020-10042-5
Kaux JF, Libertiaux V, Dupont L, Colige A, Denoël V, Lecut C, et al. Platelet-rich plasma (PRP) and tendon healing: comparison between fresh and frozen-thawed prp. Platelets. 2020;31(2):221-225. https://doi.org/10.1080/09537104.2019.159 5563
Beitia M, Delgado D, Mercader J, Gimeno I, Espregueira-Mendes J, Aizpurua B, et al. The effect of short-term cryopreservation on the properties and functionality of platelet-rich plasma. Platelets. 2023 dic.;34(1):2210243. https://doi.org/10.1080/09537104.2023.2210243
Freitas NPP, Silva Bd’A, Bezerra MRL, Pescini LYG, Olinda RG, de Mello-Salgueiro CC, et al. Freeze-dried platelet-rich plasma and stem cell-conditioned medium for therapeutic use in horses. J Equine Vet Sci. 2023 feb.;121:104189. https://doi.org/10.1016/j.jevs.2022.104189
Lee MI, Kim JH, Kwak HH, Woo HM, Han JH, Yayon A, et al. A placebo-controlled study comparing the efficacy of intra-articular injections of hyaluronic acid and a novel hyaluronic acid-platelet-rich plasma conjugate in a canine model of osteoarthritis. J Orthop Surg Res. 2019 sep. 18;14(1):314. https://doi.org/10.1186/S13018-019-1352-1
Perego R, Spada E, Moneta E, Baggiani L, Proverbio D. Use of autologous leucocyte- and platelet-rich plasma (l-PRP) in the treatment of aural hematoma in dogs. Vet Sci. 2021 ag. 28;8(9):172. https://doi.org/10.3390/vetsci8090172
Agostinho-Alves JCA, Pereira dos Santos AMM, Figuereido-Jorge PI, Branco-Lavrador, CFT, Alves-Carreira LM. Management of osteoarthritis using 1 intra-articular platelet concentrate admi-nistration in a canine osteoarthritis model. Am J Sports Med. 2021 mar.;49(3):599-608. https://doi.org/10.1177/0363546520981558
AbdEl-Raouf M, Wang X, Miusi S, Chai J, AbdEl-Aal ABM, Nefissa-Helmy MM, et al. Injectable-platelet rich fibrin using the low-speed centrifugation concept improves cartilage regeneration when compared to platelet-rich plasma. Platelets. 2019;30(2):213-221. https://doi.org/10.1080/09537104.2017.1401058
Lee JB, Lee JT, Hwang S, Choi JY, Rhyu IC, Yeo ISL. Leukocyte -and platelet-rich fibrin is an effective membrane for lateral ridge augmentation: An in vivo study using a canine model with surgically created defects. J Periodontol. 2020 ene.;91(1):120-128. https://doi.org/10.1002/JPER.19-0186
Gupta N, Agarwal S. Advanced–PRF: Clinical evaluation in impacted mandibular third molar sockets. J Stomatol Oral Maxillofac Surg. 2021 feb.;122(1):43-49. https://doi.org/10.1016/j.jormas.2020.04.008
To M, Su CY, Hidaka K, Okudera T, Matsuo M. Effect of advanced platelet-rich fibrin on accelerating alveolar bone formation in dogs: a histological and immunofluorescence evaluation. Anat Sci Int. 2019 jun.;94(3):238-244. https://doi.org/10.1007/s12565-019-00479-1
Behnaz M, Izadi SS, Mashhadi-Abbas F, Dianat O, Sadeghabadi S, Akbarzadeh T, et al. The impact of platelet-rich fibrin (PRF) on delayed tooth replantation: A preliminary animal study. Aust Endod J. 2021 dic.;47(3):457-466. https://doi.org/10.1111/aej.12492
Soares CS, Dias IR, Pires, MA, Carvalho PP. Canine-origin platelet-rich fibrin as an effective bio-material for wound healing in domestic cats: A preliminary study. Vet Sci. 2021 sep. 30;8(10):213. https://doi.org/10.3390/vetsci8100213
Aydinyurt HS, Sancak T, Taskin C, Basbugan Y, Akinci L. Effects of injectable platelet-rich fibrin in experimental periodontitis in rats. Odontology. 2021 abr.;109(2):422-432. https://doi.org/10.1007/S10266-020-00557-1
Miguel-Pastor L, Satué K, Chicharro D, Peláez P, Torres-Torrillas M, Carrillo JM, et al. Evaluation of platelet-rich plasma by means of PRGF®-Endoret® protocol in leukemia cats: PDGF-BB and TGF-β1 valuation. Front Vet Sci. 2023 en. 26;10:1110055. https://doi.org/10.3389/fvets.2023.1110055
Castilho MS, Rahal SC, Dias-Neto R das N, Pereira AC, de Almeida-Francia CCD, Reis-Mesquita, L, et al. Preparation and characterization of leukocyte- and platelet-rich fibrin membrane derived from cats’ blood. Microsc Res Tech. 2021 ag.;84(8):1802-1808. https://doi.org/10.1002/jemt.23737
Caterino C, Della Valle G, Aragosa F, De Biase D, Ferrara G, Lamagna F, et al. (2022). Production protocol standardization, macroscopic and histological evaluation, and growth factor quantification of canine leukocyte-and platelet-rich fibrin membranes. Front Vet Sci. 2022 abr. 15;9:861255. https://doi.org/10.3389/fvets.2022.861255
Mourad SI, Al-Dubai SA, Elsayed, SA, El-Zehary RR. Efficacy of platelet-rich fibrin and tacrolimus on facial nerve regeneration: An animal study. Int J Oral Maxillofac Surg. 2022 feb.;51(2):279-287. https://doi.org/10.1016/j.ijom.2021.05.016
Kobayashi E, Flückiger L, Fujioka-Kobayashi M, Sawada K, Sculean A, Schaller B, Miron RJ. Comparative release of growth factors from PRP, prf, and advanced-PRF. Clin Oral Investig. 2016 dic.;20(9):2353-2360. https://doi.org/10.1007/s00784-016-1719-1
Li S, Yang H, Duan Q , Bao H, Li A, Li W, et al. A comparative study of the effects of platelet-rich fibrin, concentrated growth factor and platelet-poor plasma on the healing of tooth extraction sockets in rabbits. BMC Oral Health. 2022 mzo. 23;22(1):87. https://doi.org/10.1186/s12903-022-02126-0
Choi SY, Kim S, Park KM. Initial healing effects of platelet-rich plasma (prp) gel and platelet-rich fibrin (prf) in the deep corneal wound in rabbits. Bioengineering. 2022 ag. 20;9(8):405. https://doi.org/10.3390/bioengineering9080405
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Derechos de autor 2026 Carlos Adrián Ospina Bolaños, Ómar Leonardo Aristizábal (Autor/a)
